Total Ketone Bodies (T-KB)
Autokit Total Ketone BodiesFor In Vitro Diagnostic Use
Intended Use
Autokit Total Ketone Bodies is an in vitro assay for the quantitative determination of total ketone bodies [acetoacetate (AcAc) + 3-hydroxybutyrate (3-HB)] in serum or plasma.
Method
Cyclic Enzymatic Method
Special Characteristics
Autokit Total Ketone Bodies is based on Cyclic Enzymatic Method without deproteinization in samples. Standard Method (measuring range 1.03-1000 μmol/L) or High Sensitivity Method (measuring range 0.16-200 μmol/L) can be selected depending on the amount of samples.
Principle of the Method
When a sample is mixed with R1 and R2, AcAc and 3-HB in the sample are converted to 3-HB and AcAc, respectively, in the presence of 3-HBDH, NADH, and Thio-NAD. 3-HB and AcAc produced in the enzymatic reactions are, then, converted to AcAc and 3-HB, respectively. During these cyclic reactions, NAD and Thio-NADH are produced. By measuring the rate of Thio-NADH production spectrophotometrically, the concentration of total ketone bodies in the sample is determined.
Standard Procedure
Application to automated analyzers and Package are available upon request
Ordering Information
| Product | Storage Condition | Shelf Life | |
|---|---|---|---|
| Autokit Total Ketone Bodies R1 Set | Thio-NAD Buffer |
2-10°C | 12 months |
| Autokit Total Ketone Bodies R2 Set | Enzyme Diluent |
2-10°C | 12 months |
| Calibrator | Storage Condition | Shelf Life |
|---|---|---|
| Ketone Body Calibrator・300 | 2-10°C | 12 months |
| Ketone Body Calibrator・40 | 2-10°C | 12 months |
