Total Ketone Bodies (T-KB)
Autokit Total Ketone BodiesFor In Vitro Diagnostic Use
Autokit Total Ketone Bodies is an in vitro assay for the quantitative determination of total ketone bodies [acetoacetate (AcAc) + 3-hydroxybutyrate (3-HB)] in serum or plasma.
Cyclic Enzymatic Method
Autokit Total Ketone Bodies is based on Cyclic Enzymatic Method without deproteinization in samples. Standard Method (measuring range 1.03-1000 μmol/L) or High Sensitivity Method (measuring range 0.16-200 μmol/L) can be selected depending on the amount of samples.
Principle of the Method
When a sample is mixed with R1 and R2, AcAc and 3-HB in the sample are converted to 3-HB and AcAc, respectively, in the presence of 3-HBDH, NADH, and Thio-NAD. 3-HB and AcAc produced in the enzymatic reactions are, then, converted to AcAc and 3-HB, respectively. During these cyclic reactions, NAD and Thio-NADH are produced. By measuring the rate of Thio-NADH production spectrophotometrically, the concentration of total ketone bodies in the sample is determined.
Application to automated analyzers and Package are available upon request
|Product||Storage Condition||Shelf Life|
|Autokit Total Ketone Bodies R1 Set||Thio-NAD
|Autokit Total Ketone Bodies R2 Set||Enzyme
|Calibrator||Storage Condition||Shelf Life|
|Ketone Body Calibrator・300||2-10°C||12 months|
|Ketone Body Calibrator・40||2-10°C||12 months|