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Urea Nitrogen (UN)

L-Type UNFor In Vitro Diagnostic Use

Intended Use

L-Type UN is an in vitro assay for the quantitative determination of UN in serum, plasma or urine.


Urease·GIDH Method

Special Characteristics

L-Type UN is not interfered significantly by other serum components.

Principle of the Method

Urea in a sample is decomposed to produce ammonia and carbon dioxice catalyzed by urease. In the presence of α-ketoglutarate and β-Nicotinamide adenine dinucleotide, reduced form (NADH), this ammonia causes the oxidation of an equal amount of NADH to NAD in a reaction catalyzed by glutamate dehydrogenase (GlDH). The decrease in absorbance of NADH at 340nm i directly proportional to the amount of urea nitrogen in the sample.

Urea Nitrogen (UN) Principle of the Method

Standard Procedure

Urea Nitrogen (UN) Standard Procedure

Application to automated analyzers and Package are available upon request

Ordering Information

Product Storage Condition Shelf Life
L-Type UN Coenzyme 2-10°C Do not freeze 12 months
L-Type UN Substrate Enzyme 2-10°C Do not freeze 12 months
Calibrator Storage Condition Shelf Life
Multi-Chem Calibrator B 2-10°C 12 months

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