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Aspartate Aminotransferase (AST)

L-Type AST.J2For In Vitro Diagnostic Use

Intended Use

L-Type AST.J2 is an in vitro assay for the quantitative determination of the activity of aspartate aminotransferase (AST) in serum or plasma.


JSCC Transferable Method

Special Characteristics

L-Type AST.J2 is a liquid reagent which improves the stable formation of coenzyme.

Principle of the Method

When a sample is mixed with Substrate-Enzyme and α-KG, glutamate and oxaloacetate are formed from L-aspartate and α-ketoglutarate in a reaction catalyzed by AST in the sample. The oxaloacetate thus produced is converted to malate by malate dehydrogenase (MDH) and at the same time, NADH is oxidized to NAD. By measuring the decrease in absorbance at 340 nm due to the oxidation of NADH, AST activity in the sample is determined.

Aspartate Aminotransferase (AST) Principle of the Method

Standard Procedure

Aspartate Aminotransferase (AST) Standard Procedure

Application to automated analyzers and Package are available upon request

Ordering Information

Product Storage Condition Shelf Life
L-Type AST.J2 Substrate-Enzyme 2-10°C 12 months
L-Type AST.J2 α-KG 2-10°C 18 months
Calibrator Storage Condition Shelf Life
Enzyme Calibrator 8°C or lower 10 months

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