Amylase (AMY)
L-Type AmylaseFor In Vitro Diagnostic Use
Intended Use
L-Type Amylase is an in vitro assay for the quantitative determination of the amylase activity in serum, plasma or urine.
Method
JSCC·IFCC Transferable Method
Special Characteristics
L-Type Amylase uses BG5P as substrate due to no interferences from glucoamylase and aglucosidase.
The substrate structure and reaction product are clear.
Principle of the Method
When a specimen is allowed to react with the BG5P, BG3 and p-nitrophenyl α-maltoside are formed by the action of α-amylase in the specimen. Thus p-nitrophenyl α-maltoside produced is hydrolyzed to p-nitrophenol by the reactions of both glucoamylase and α-glucosidase. By measuring the increase in absorbance due to the production of p-nitrophenol, α-amylase activity in the specimen is determined. A benzyl group in the nonreducing end glucose residue of BG5P prevents the hydrolysis by the coupled enzymes and results in giving low blank. Also, one unit of BG5P hydrolyzed by α-amylase converts into one p-nitrophenol molecule. Therefore, one unit of α-amylase can be defined stoichiometrically as the amount of enzyme that produces 1 micro mol of p-nitrophenol per minute.
Standard Procedure
Application to automated analyzers and Package are available upon request
Ordering Information
| Product | Storage Condition | Shelf Life | |
|---|---|---|---|
| L-Type Amylase | Enzyme | 2-10°C | 12 months |
| L-Type Amylase | Substrate | 2-10°C | 12 months |
| Calibrator | Storage Condition | Shelf Life |
|---|---|---|
| Enzyme Calibrator | 8°C or lower | 10 months |
