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Amylase (AMY)

L-Type AmylaseFor In Vitro Diagnostic Use

Intended Use

L-Type Amylase is an in vitro assay for the quantitative determination of the amylase activity in serum, plasma or urine.


JSCC·IFCC Transferable Method

Special Characteristics

L-Type Amylase uses BG5P as substrate due to no interferences from glucoamylase and aglucosidase.
The substrate structure and reaction product are clear.

Principle of the Method

When a specimen is allowed to react with the BG5P, BG3 and p-nitrophenyl α-maltoside are formed by the action of α-amylase in the specimen. Thus p-nitrophenyl α-maltoside produced is hydrolyzed to p-nitrophenol by the reactions of both glucoamylase and α-glucosidase. By measuring the increase in absorbance due to the production of p-nitrophenol, α-amylase activity in the specimen is determined. A benzyl group in the nonreducing end glucose residue of BG5P prevents the hydrolysis by the coupled enzymes and results in giving low blank. Also, one unit of BG5P hydrolyzed by α-amylase converts into one p-nitrophenol molecule. Therefore, one unit of α-amylase can be defined stoichiometrically as the amount of enzyme that produces 1 micro mol of p-nitrophenol per minute.

Amylase (AMY) Principle of the Method

Standard Procedure

Amylase (AMY) Standard Procedure

Application to automated analyzers and Package are available upon request

Ordering Information

Product Storage Condition Shelf Life
L-Type Amylase Enzyme 2-10°C 12 months
L-Type Amylase Substrate 2-10°C 12 months
Calibrator Storage Condition Shelf Life
Enzyme Calibrator 8°C or lower 10 months

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