L-Type AmylaseFor In Vitro Diagnostic Use
L-Type Amylase is an in vitro assay for the quantitative determination of the amylase activity in serum, plasma or urine.
JSCC·IFCC Transferable Method
L-Type Amylase uses BG5P as substrate due to no interferences from glucoamylase and aglucosidase.
The substrate structure and reaction product are clear.
Principle of the Method
When a specimen is allowed to react with the BG5P, BG3 and p-nitrophenyl α-maltoside are formed by the action of α-amylase in the specimen. Thus p-nitrophenyl α-maltoside produced is hydrolyzed to p-nitrophenol by the reactions of both glucoamylase and α-glucosidase. By measuring the increase in absorbance due to the production of p-nitrophenol, α-amylase activity in the specimen is determined. A benzyl group in the nonreducing end glucose residue of BG5P prevents the hydrolysis by the coupled enzymes and results in giving low blank. Also, one unit of BG5P hydrolyzed by α-amylase converts into one p-nitrophenol molecule. Therefore, one unit of α-amylase can be defined stoichiometrically as the amount of enzyme that produces 1 micro mol of p-nitrophenol per minute.
Application to automated analyzers and Package are available upon request
|Product||Storage Condition||Shelf Life|
|L-Type Amylase||Enzyme||2-10°C||12 months|
|L-Type Amylase||Substrate||2-10°C||12 months|
|Calibrator||Storage Condition||Shelf Life|
|Enzyme Calibrator||8°C or lower||10 months|