Creatine Phosphokinase (CPK)

Intended Use

L-Type CK is an in vitro assay for the quantitative determination of the activity of creatine phosphokinase (CPK) in serum or plasma.

Method

JSCC Transferable Method

Special Characteristics

L-Type CK is a stable reagent due to the use of Thioglycerol as activator instead of N-acetyl-Lcysteine (NAC) whose oxidized products inhibit CK activity. This reagent using double kinetic mode measurement is not interfered significantly by Myokinase in hemolysis.

Principle of the Method

When a specimen is mixed with reagent, containing creatine phosphate (CP), adenosine-5'-diphosphate (ADP), glucose, hexokinase (HK) and glucose-6-phosphate dehydrogenase (G-6-PDH), ADP is converted to adenosine-5'-triphoaphate (ATP) by the action of CK in the specimen.
ATP so formed is used to synthesize Glucose-6-phosphate (G-6-P) in a reaction catalyzed by HK and glucose in the reagent. G-6-P, produced in this way, is oxidized to 6-phosphogluconate in a reaction catalyzed by G-6-PDH, while Nicotinamide adenine dinucleotide phosphate (NADP) is reduced to NADPH. The NADPH formed is measured by determining its absorbance at 340 nm and this is directly proportional to the amount of CK activity in the specimen.

Standard Procedure

Application to automated analyzers and Package are available upon request

Ordering Information