Direct Bilirubin (D-Bil)
Direct Bilirubin E-HAFor In Vitro Diagnostic Use
Intended Use
Direct Bilirubin E-HA is an in vitro assay for the quantitative determination of direct bilirubin in serum or plasma.
Method
Vanadate oxidation method
Special Characteristics
Hemolysis may give negative influence on thte assay. Direct Bilirubin E-HA is not interfered significantly by other serum components and hemoglobin (up to 500 mg/dL). This reagent is highly precise, stable and a convenient ready-to-use liquid reagent, utilizing vanadate as oxidizing agent.
Principle of the Method
When a sample is mixed with the reagent containing the metavanadate at around pH 3, direct bilirubin in the sample is oxidized to biliverdine. Then the absorbance of yellow specific to bilirubin decreases. Therefore, the direct bilirubin concentration in the sample can be obtained by measuring the absorbance before and after the metavanadate oxidation.
Standard Procedure
Application to automated analyzers and Package are available upon request
Ordering Information
| Product | Storage Condition | Shelf Life | |
|---|---|---|---|
| Direct Bilirubin E-HA | Buffer | 2-10°C | 12 months |
| Direct Bilirubin E-HA | Vanadate | 2-35°C | 18 months |
| Calibrator | Storage Condition | Shelf Life |
|---|---|---|
| Bilirubin Calibrator | 2-10°C | 18 months |
