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Direct Bilirubin (D-Bil)

Direct Bilirubin E-HAFor In Vitro Diagnostic Use

Intended Use

Direct Bilirubin E-HA is an in vitro assay for the quantitative determination of direct bilirubin in serum or plasma.


Vanadate oxidation method

Special Characteristics

Hemolysis may give negative influence on thte assay. Direct Bilirubin E-HA is not interfered significantly by other serum components and hemoglobin (up to 500 mg/dL). This reagent is highly precise, stable and a convenient ready-to-use liquid reagent, utilizing vanadate as oxidizing agent.

Principle of the Method

When a sample is mixed with the reagent containing the metavanadate at around pH 3, direct bilirubin in the sample is oxidized to biliverdine. Then the absorbance of yellow specific to bilirubin decreases. Therefore, the direct bilirubin concentration in the sample can be obtained by measuring the absorbance before and after the metavanadate oxidation.

Direct Bilirubin (D-Bil) Principle of the Method

Standard Procedure

Direct Bilirubin (D-Bil) Standard Procedure

Application to automated analyzers and Package are available upon request

Ordering Information

Product Storage Condition Shelf Life
Direct Bilirubin E-HA Buffer 2-10°C 12 months
Direct Bilirubin E-HA Vanadate 2-35°C 18 months
Calibrator Storage Condition Shelf Life
Bilirubin Calibrator 2-10°C 18 months

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