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Phospholipids (PL)

L-Type Phospholipids For In Vitro Diagnostic Use

Intended Use

L-Type Phospholipids is an in vitro assay for the quantitative determination of phospholipids in serum or plasma.


Choline oxidase・DAOS Method

Special Characteristics

L-Type PL based on Choline oxidase・DAOS method is not interfered significantly by other serum components. This is a liquid type reagent which is stable to be designed for general biochemical automated analyzer.

Principle of the Method

Phospholipids (lecithin, lysolecithin and sphingomyelin) in the sample are hydrolyzed to choline by phospholipase D. The choline thus produced is oxidized to betaine by choline oxidase yielding hydrogen peroxide. In the presence of peroxidase (POD), the hydrogen peroxide formed yields a blue pigment by quantitative oxidation condensation with DAOS and 4-aminoantipyrine. The phospholipids concentration is obtained by measuring the absorbance of the blue color.

Phospholipids (PL) Standard Procedure

Standard Procedure

Phospholipids (PL) Standard Procedure

Ordering Information

Product Storage Condition Shelf Life
L-Type Phospholipids Enzyme Color A 2-10°C 12 months
L-Type Phospholipids Enzyme Color B 2-10°C 12 months
Calibrator Storage Condition Shelf Life
Multi Calibrator Lipids 2-10°C 12 months

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