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Alkaline Phosphatase (ALP)

L-Type ALP-J2For In Vitro Diagnostic Use

Intended Use

L-Type ALP-J2 is an in vitro assay for the quantitative determination of the activity of alkaline phosphatase (ALP) in serum or plasma.

Method

JSCC Transferable Method

Special Characteristics

L-Type ALP-J2 is not interfered significantly by other serum components such as high bilirubin in samples. The stability of this reagent after preparation is improved compared with conventional one.

Principle of the Method

When a sample is mixed with 2-(ethylamino)ethanol (EAE) buffer, Alkaline phosphatase (ALP) reacts with 4-nitrophenylphosphate and liberates 4-nitrophenol. By measuring the formation rate of 4-nitrophenol, ALP activity in the sample is determined.

Alkaline Phosphatase (ALP) Principle of the Method

Standard Procedure

Alkaline Phosphatase (ALP) Standard Procedure

Application to automated analyzers and Package are available upon request

Ordering Information

Product Storage Condition Shelf Life
L-Type ALP-J2 Buffer 2-10°C 12 months
L-Type ALP-J2 Substrate 2-10°C 12 months
Calibrator Storage Condition Shelf Life
Enzyme Calibrator 8°C or lower 10 months

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