Pancreatic Amylase (P-AMY)
L-Type P-AMYFor In Vitro Diagnostic Use
Intended Use
L-Type P-AMY is an in vitro assay for the quantitative determination of the amylase isozyme in serum, plasma or urine.
Method
Immunoinhibition Method
Special Characteristics
L-Type P-AMY uses BG5P as the substrate which is not hydrolyzed by glucoamylase and α-glucosidase. The substrate is stoichiometrically converted to the reaction product. S-amylase activity is inhibited specifically by monoclonal antibody.
Principle of the Method
When anti-S amylase antibody reacts with S-amylase, S-amylase activity is specifically inhibited. A specimen is allowed to react with the BG5P, BG3 and p-nitrophenyl α-maltoside are formed by the action of p-amylase in the specimen. Thus p-nitrophenyl α-maltoside produced is hydrolyzed to p-nitrophenol by the reactions of both glucoamylase and α-glucosidase. By measuring the increase in absorbance due to the production of p-nitrophenol, p-amylase activity in the specimen is determined. A benzyl group in the non-reducing end glucose residue of BG5P prevents the hydrolysis by the coupling enzymes and results in giving low blank. Also, one unit of BG5P hydrolyzed by p-amylase is converted to one p-nitrophenol molecule. Therefore, one unit of α-amylase can be defined stoichiometrically as the amount of enzyme that produces 1 micro mol of p-nitrophenol per minute.
Standard Procedure
Ordering Information
| Product | Storage Condition | Shelf Life |
|---|---|---|
| L-Type P-AMY Antibody Enzyme | 2-10℃ (Do not freeze) | 12 months |
| L-Type P-AMY Substrate | 2-10℃ (Do not freeze) | 12 months |
| Calibrator | Storage Condition | Shelf Life |
|---|---|---|
| Enzyme Calibrator | 8°C or lower | 10 months |
