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Creatinine (CRE)

L-Type Creatinine MFor In Vitro Diagnostic Use

Intended Use

L-Type Creatinine M is an in vitro assay for the quantitative determination of creatinine in serum, plasma or urine.


Creatininase-HMMPS Method

Special Characteristics

L-Type Creatinine M is based on Creatininase-HMMPS method using color former HMMPS (N-(3-sulfopropyl)-3-methoxy-5-methylaniline) which Wako developed. This reagent is not interfered significantly by other serum components and catecholamines.

Principle of the Method

When a sample is mixed with Enzyme Color A and Enzyme Color B, creatinine in the sample is converted to creatine by the action of creatininase. The creatine formed is hydrolyzed by creatinase to product sarcosine and urea. The sarcosine produced is then decomposed by sarcosine oxidase to form glycine, formaldehyde and hydrogen peroxide. In the presence of peroxidase (POD), then hydrogen peroxide formed yields a blue pigment by quantitative oxidation condensation with N-(3-sulfopropyl)-3-methoxy-5-methylaniline (HMMPS) and 4-aminoantipyrine.
The creatinine concentration is obtained by measuring the absorbance of blue color.

Creatinine (CRE) Principle of the Method

Standard Procedure

Creatinine (CRE) Standard Procedure

Application to automated analyzers and Package are available upon request

Ordering Information

Product Storage Condition Shelf Life
L-Type Creatinine M Enzyme Color A 2-10°C 24 months
L-Type Creatinine M Enzyme Color B 2-10°C 24 months
Calibrator Storage Condition Shelf Life
Multi-Chem Calibrator A 2-10°C 12 months

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