3-hydroxybutyrate (3-HB)
Autokit 3-HBFor In Vitro Diagnostic Use
Intended Use
Autokit 3-HB is an in vitro assay for the quantitative determination of 3-hydroxybutyrate (3-HB) in serum or plasma.
Method
Cyclic Enzymatic Method
Special Characteristics
Autokit 3-HB is based on Cyclic Enzymatic Method without deproteinization in samples. Standard Method (measuring range 1.17-1000μmol/L) or High Sensitivity Method (measuring range 0.15-200 μmol/L) can be selected depending on the amount of samples.
Principle of the Method
When a sample is mixed with R1, AcAc in the sample is broken down to acetone by AADC.
Upon addition of R2, 3-HB in the sample is oxidized in the presence of 3-HBDH and Thio-NAD. This oxidation triggers the cyclic reactions. Since the original AcAc in the sample has been removed, only 3-HB is assayed by measuring the rate of Thio-NADH production spectrophotometrically.
Standard Procedure
*In the case of high sensitivity method, sample volume is 17 uL.
Calibrator : Ketone Body Calibrator・300 or Ketone Body Calibrator・40 (Available separately)
Application to automated analyzers and Package are available upon request
Ordering Information
| Product | Storage Condition | Shelf Life | |
|---|---|---|---|
| Autokit 3-HB R1 Set | Thio-NAD Buffer |
2-10°C (Do not freeze) | 12 months |
| Autokit 3-HB R2 Set | Enzyme Diluent |
2-10°C | 12 months |
| Calibrator | Storage Condition | Shelf Life |
|---|---|---|
| Ketone Body Calibrator・300 | 2-10°C | 12 months |
| Ketone Body Calibrator・40 | 2-10°C | 12 months |
