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3-hydroxybutyrate (3-HB)

Autokit 3-HBFor In Vitro Diagnostic Use

Intended Use

Autokit 3-HB is an in vitro assay for the quantitative determination of 3-hydroxybutyrate (3-HB) in serum or plasma.


Cyclic Enzymatic Method

Special Characteristics

Autokit 3-HB is based on Cyclic Enzymatic Method without deproteinization in samples. Standard Method (measuring range 1.17-1000μmol/L) or High Sensitivity Method (measuring range 0.15-200 μmol/L) can be selected depending on the amount of samples.

Principle of the Method

When a sample is mixed with R1, AcAc in the sample is broken down to acetone by AADC.

3-hydroxybutyrate (3-HB) Principle of the Method

Upon addition of R2, 3-HB in the sample is oxidized in the presence of 3-HBDH and Thio-NAD. This oxidation triggers the cyclic reactions. Since the original AcAc in the sample has been removed, only 3-HB is assayed by measuring the rate of Thio-NADH production spectrophotometrically.

Total Ketone Bodies (T-KB) Principle of the Method

Standard Procedure

3-hydroxybutyrate (3-HB) Standard Procedure

*In the case of high sensitivity method, sample volume is 17 uL.
Calibrator : Ketone Body Calibrator・300 or Ketone Body Calibrator・40 (Available separately)

Application to automated analyzers and Package are available upon request

Ordering Information

Product Storage Condition Shelf Life
Autokit 3-HB R1 Set Thio-NAD
2-10°C (Do not freeze) 12 months
Autokit 3-HB R2 Set Enzyme
2-10°C 12 months
Calibrator Storage Condition Shelf Life
Ketone Body Calibrator・300 2-10°C 12 months
Ketone Body Calibrator・40 2-10°C 12 months

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