Lipase
Autokit LipaseFor In Vitro Diagnostic Use
Intended Use
Autokit Lipase is an in vitro assay for the quantitative determination of the activity of Lipase in serum.
Method
1,2-diglyceride Substrate·TOOS Method
Special Characteristics
Autokit Lipase is based on color rate method, which utilizes 1, 2-diglyceride as substrate. This reagent is easy to prepare and stable for 10 days at 2-10°C.
Principle of the Method
When a sample is added to the natural type of 1, 2-diglyceride, it is separated to fatty acids and 2-monoglyceride by pancreatic lipase in sample. The 2-monoglyceride is hydrolyzed to glycerol and fatty acids by monoglyceride lipase (MGLP).The glycerol is phosphorylated by glycerol kinase (GK) and is oxidized by glycerol-3-phosphate oxidase (GPO) yielding hydrogen peroxide. The hydrogen peroxide formed causes TOOS and 4-aminoantipyrine (4-AA) to undergo a quantitative oxidative condensation catalyzed by peroxidase (POD), producing a bluish purple pigment. The activity of lipase in the sample is determined by measuring the absorbance of the bluish purple color.
![lipase Principle of the Method](https://diagnostic-wako.fujifilm.com/asia/assets/images/product/19_01.png)
Standard Procedure
![lipase Standard Procedure](https://diagnostic-wako.fujifilm.com/asia/assets/images/product/19_02.png)
Application to automated analyzers and Package are available upon request
Ordering Information
Product | Storage Condition | Shelf Life | |
---|---|---|---|
Autokit Lipase | Enzyme Substrate | 2-10°C | 12 months |
Diluent | 2-10°C | 12 months | |
Standard Solution | 2-10°C | 12 months |