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Autokit LipaseFor In Vitro Diagnostic Use

Intended Use

Autokit Lipase is an in vitro assay for the quantitative determination of the activity of Lipase in serum.


1,2-diglyceride Substrate·TOOS Method

Special Characteristics

Autokit Lipase is based on color rate method, which utilizes 1, 2-diglyceride as substrate. This reagent is easy to prepare and stable for 10 days at 2-10°C.

Principle of the Method

When a sample is added to the natural type of 1, 2-diglyceride, it is separated to fatty acids and 2-monoglyceride by pancreatic lipase in sample. The 2-monoglyceride is hydrolyzed to glycerol and fatty acids by monoglyceride lipase (MGLP).The glycerol is phosphorylated by glycerol kinase (GK) and is oxidized by glycerol-3-phosphate oxidase (GPO) yielding hydrogen peroxide. The hydrogen peroxide formed causes TOOS and 4-aminoantipyrine (4-AA) to undergo a quantitative oxidative condensation catalyzed by peroxidase (POD), producing a bluish purple pigment. The activity of lipase in the sample is determined by measuring the absorbance of the bluish purple color.

lipase Principle of the Method

Standard Procedure

lipase Standard Procedure

Application to automated analyzers and Package are available upon request

Ordering Information

Product Storage Condition Shelf Life
Autokit Lipase Enzyme Substrate 2-10°C 12 months
Diluent 2-10°C 12 months
Standard Solution 2-10°C 12 months

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