Lactate Dehydrogenase (LD)
L-Type LD.JFor In Vitro Diagnostic Use
Intended Use
L-Type LD.J is an in vitro assay for the quantitative determination of the activity of lactate dehydrogenase (LD) in serum or plasma.
Method
UV kinetic (JSCC Transferable Method)
Special Characteristics
L-Type LD.J is a liquid type reagent which is stable to be designed for general biochemical automated analyzer.
Principle of the Method
When a sample is mixed with the reagent, lactate is oxidized to pyruvate in the presence of NAD in diethanolamine buffer. NAD is reduced to β-nicotinamide adenine dinucleotide, reduced form (NADH) resulting in the increase of absorbance at 340 nm. By measuring the formation rate of NADH, LD activity in the sample is determined.
Standard Procedure
Application to automated analyzers and Package are available upon request
Ordering Information
| Product | Storage Condition | Shelf Life |
|---|---|---|
| L-Type LD.J Substrate Buffer | 2-25°C | 18 months |
| L-Type LD.J NAD | 2-10°C | 12 months |
| Calibrator | Storage Condition | Shelf Life |
|---|---|---|
| Enzyme Calibrator | 8°C or lower | 10 months |
