Triglycerides (TG)
L-Type Triglyceride MFor In Vitro Diagnostic Use
Intended Use
L-Type Triglyceride M is an in vitro assay for the quantitative determination of triglyceride in serum or plasma.
Method
GPO-HMMPS, Glycerol blanking Method
Special Characteristics
L-Type Triglyceride M is not interfered significantly from other serum components thanks to high ability to remove turbidity. This reagent shows superior linearity in broad range (1.1-2000 mg/dL) because of highly reactive lipoprotein lipase (LPL). This is based on GPO-HMMPS method using color former HMMPS (N-(3-sulfopropyl)-3-methoxy-5-methylaniline) which Wako developed.
Principle of the Method
First reaction (Elimination of free glycerol)
Free glycerol in specimen is eliminated by the action of glycerolkinase (GK), glycerol-3-phosphate oxidase (GPO) and catalase.
![Triglycerides (TG) Principle of the Method](https://diagnostic-wako.fujifilm.com/asia/assets/images/product/23_01.png)
Second reaction (Triglyceride measurement)
Triglyceride in specimen is converted to dihydroxyacetone phosphate and hydrogen peroxide by the action of lipoprotein lipase (LPL), glycerolkinase (GK) and glycerol-3-phosphate oxidase (GPO). The reaction goes on as follows. In the presence of peroxidase (POD), the hydrogen peroxide formed yields a blue pigment by quantitative oxidation condensation with N-(3-sulfopropyl)-3-methoxy-5-methylaniline (HMMPS) and 4-aminoantipyrine. The triglyceride concentration is obtained by measuring the absorbance of the blue color.
![Triglycerides (TG) Principle of the Method](https://diagnostic-wako.fujifilm.com/asia/assets/images/product/23_02.png)
Standard Procedure
![Triglycerides (TG) Standard Procedure](https://diagnostic-wako.fujifilm.com/asia/assets/images/product/23_03.png)
Application to automated analyzers and Package are available upon request
Ordering Information
Product | Storage Condition | Shelf Life | |
---|---|---|---|
L-Type Triglyceride M | Enzyme Color A | 2-10°C | 24 months |
L-Type Triglyceride M | Enzyme Color B | 2-10°C | 24 months |
Calibrator | Storage Condition | Shelf Life |
---|---|---|
Multi Calibrator Lipids | 2-10°C | 12 months |