Triglycerides (TG)

Intended Use

L-Type Triglyceride M is an in vitro assay for the quantitative determination of triglyceride in serum or plasma.

Method

GPO-HMMPS, Glycerol blanking Method

Special Characteristics

L-Type Triglyceride M is not interfered significantly from other serum components thanks to high ability to remove turbidity. This reagent shows superior linearity in broad range (1.1-2000 mg/dL) because of highly reactive lipoprotein lipase (LPL). This is based on GPO-HMMPS method using color former HMMPS (N-(3-sulfopropyl)-3-methoxy-5-methylaniline) which Wako developed.

Principle of the Method

First reaction (Elimination of free glycerol)

Free glycerol in specimen is eliminated by the action of glycerolkinase (GK), glycerol-3-phosphate oxidase (GPO) and catalase.

Second reaction (Triglyceride measurement)

Triglyceride in specimen is converted to dihydroxyacetone phosphate and hydrogen peroxide by the action of lipoprotein lipase (LPL), glycerolkinase (GK) and glycerol-3-phosphate oxidase (GPO). The reaction goes on as follows. In the presence of peroxidase (POD), the hydrogen peroxide formed yields a blue pigment by quantitative oxidation condensation with N-(3-sulfopropyl)-3-methoxy-5-methylaniline (HMMPS) and 4-aminoantipyrine. The triglyceride concentration is obtained by measuring the absorbance of the blue color.

Standard Procedure

Application to automated analyzers and Package are available upon request

Ordering Information