HDL Cholesterol (HDL-C)

Intended Use

L-Type HDL-C M (3) is an in vitro assay for the quantitative determination of high density lipoprotein cholesterol (HDL-C) in serum or plasma.

Method

Selective elimination method

Special Characteristics

L-Type HDL-C M (3) is based on Selective elimination method which measures HDL-C specifically, showing excellent correlation with CDC reference method (Designated Comparison method). This reagent is convenient ready-to-use liquid type reagent which utilizes color former HMMPS (N-(3-sulfopropyl)-3-methoxy-5-methylaniline) which Wako developed.

Principle of the Method

First reaction (Elimination of non-HDL cholesterol)

The block polymer containing hydrophilic and hydrophobic moiety in Pretreatment selectively binds to HDL in the specimens, and protects it from enzyme (CHE, CO) reactions. CHE and CO react with non-HDL lipoprotein [chylomicron (CM), very low density lipoprotein (VLDL), low density lipoprotein (LDL)]. Hydrogen peroxide produced by the enzyme reactions with non-HDL cholesterol is decomposed to water by catalase in Pretreatment reagent.

Second reaction (Color reaction of HDL cholesterol)

When Reacting Solution is added, the cholesterol and its derivatives in HDL produce hydrogen peroxide by CHE and CO. Hydrogen peroxide produced by the enzyme reactions with HDL-cholesterol yields a blue color pigment upon oxidative condensation with N-(3-sulfopropyl)-3-methoxy-5-methylaniline (HMMPS) and 4-animoantipyrine in the presence of peroxidase (POD). The amount of HDL-cholesterol contained in the sample is determined by measuring the absorbance of the blue color.

Standard Procedure

Application to automated analyzers and Package are available upon request

Ordering Information