Total Cholesterol (T-Cho)
L-Type CHO MFor In Vitro Diagnostic Use
Intended Use
L-Type CHO M is an in vitro assay for the quantitative determination of total cholesterol in serum or plasma.
Method
Cholesterol oxidase-HMMPS Method
Special Characteristics
L-Type CHO M is not interfered significantly by other serum components such as high TG samples thanks to improving the ability of removing chyle. This reagent shows high correlation with CDC Reference method (Abell-Kendall method).
Principle of the Method
When a sample is added to the Enzyme Color A and B, cholesterol esters in the sample are hydrolyzed to free cholesterol and fatty acids by cholesterol esterase. The cholesterol thus produced and the free cholesterol already present in the sample, are oxidized by cholesterol oxidase (CO) yielding hydrogen peroxide. The hydrogen peroxide formed causes N-(3-sulfopropyl)-3-methoxy-5-methylaniline (HMMPS) and 4-aminoantipyrine (4-AA) to undergo quantitative oxidative condensation catalyzed by peroxidase (POD), producing a blue pigment. The amount of total cholesterol contained in the sample is determined by measuring the absorbance of the blue color.
Standard Procedure
Application to automated analyzers and Package are available upon request
Ordering Information
| Product | Storage Condition | Shelf Life | |
|---|---|---|---|
| L-Type CHO M | Enzyme Color A | 2-10°C | 24 months |
| L-Type CHO M | Enzyme Color B | 2-10°C | 24 months |
| Calibrator | Storage Condition | Shelf Life |
|---|---|---|
| Multi Calibrator Lipids | 2-10°C | 12 months |
